New Study on SLU-PP-332

Happy Thursday!

If you’re obsessed with staying ahead of the curve and pushing the limits of what’s possible for muscle, mitochondria, and longevity…today’s deep-dive will knock your socks off.

Why?

Because a brand new study has just dropped on a molecule I’ve been raving about behind the scenes for almost a year now: SLU-PP-332.

If you’ve ever fantasized about harnessing the biochemical benefits of elite-level training…without spending hours grinding it out in the gym…this is the closest we’ve come to exercise in a capsule.

Grab your notepad, because I’m breaking down the science, the mind-blowing numbers, what you can expect, and how I’m using it personally.

Here’s a link to the new study.

What Did the Study Evaluate?

Researchers from Italy tackled a question that many of us have on our minds.

What if you could reprogram aged, sedentary human muscle cells to behave like those of a trained athlete, using nothing but a molecule?

They focused on SLU-PP-332, a first-in-class “pan-agonist” of estrogen-related receptors (ERRα/β/γ).

These are the same nuclear receptors that get switched on during hardcore training, fueling mitochondrial biogenesis, fat oxidation, muscle fiber transformation, and the metabolic rewiring that makes athletes functionally younger at the cellular level.

The scientists took muscle stem cells (myoblasts) from two groups:

• Elderly, sedentary women (the “inactive” group)

• Age-matched, lifelong exercisers (the “active” group)

They then incubated the “inactive” muscle cells with SLU-PP-332 for 48 hours at a concentration of 1 × 10⁻⁵ M (that’s 2.903 µg per mL).

The mission:

1. See if SLU-PP-332 could “wake up” inactive muscle cells

2. Measure changes in key markers of mitochondrial health, oxidative stress, muscle differentiation, and cell survival

3. Compare those results to what’s naturally seen in the “active” group

What Did the Study Find?

If you geek out over data, this is where things get wild.

SLU-PP-332 literally reprogrammed aged, sedentary muscle cells back to a youthful, exercise-adapted state. 

Check out these percentage-based improvements in just 48 hours:

1. Lower Cytotoxicity:

• LDH (cell stress marker) dropped 16.1% compared to controls.

• Translation: SLU-PP-332 made these cells less fragile, like they’d been training for months, not sitting on the couch.

2. Antioxidant Power:

• Intracellular reactive oxygen species (ROS) dropped a whopping 37.7%.

• Reduced glutathione (GSH) more than doubled, up 117.4% above control.

• Bottom line: SLU-PP-332 supercharged the muscle’s defenses.

3. Youthful Cell Behavior:

• Senescent cell markers (SA-β-gal) dropped 26.1%, meaning fewer “zombie cells” clogging up the muscle.

• This means less cellular junk, more functional tissue.

4. Mitochondrial & Survival Genes Restored:

Four absolutely crucial genes (ALL shut down by inactivity and aging) were restored to “active athlete” levels:

• SIRT1, PGC-1α, ERRα, and FNDC5 all rose to, or above, the levels found in lifelong exercisers.

• Inactive muscle had SIRT1 at just 30% of active, but SLU-PP-332 brought it right back up to 100%.

• Same with FNDC5, the gene that encodes irisin (the “exercise hormone”).

• Bcl-2 (anti-apoptosis) doubled, and Akt (cell growth) was restored to youthful values.

5. Muscle Formation (Myogenesis):

• Inactive cells treated with SLU-PP-332 developed abundant, robust, multinucleated myotubes, the exact structure found in youthful, strong muscle.

Summary:

In other words, SLU-PP-332 transformed the very biology of sedentary, aged muscle into something you’d expect in an athlete.

What Can You Expect From Taking SLU-PP-332?

Let’s bring this science out of the petri dish and into real life.

Here’s what you can reasonably expect if you implement SLU-PP-332 into your protocol, especially if you’re already training, optimizing hormones, and dialing in your diet:

1. Enhanced Mitochondrial Output:

If you’re looking for cleaner, more sustainable energy (not just a stim boost), you can expect a bump in mitochondrial function and cellular energy.

This translates into improved endurance, faster recovery, and even enhanced fat loss over time.

2. Reduced Muscle Inflammation & Fatigue:

SLU-PP-332’s antioxidant effects mean less post-training soreness, faster recovery, and possibly even protection against the “overtraining” wall.

The study showed a direct reduction in cytotoxic stress and ROS, which are two key culprits behind muscle fatigue and delayed-onset muscle soreness.

3. Muscle Growth Potential:

You won’t “grow” muscle by sitting still. But combine SLU-PP-332 with a smart training plan, and you could see enhanced differentiation and maturation of muscle fibers.

Your efforts in the gym count for more, and “recomp” (simultaneous fat loss and muscle gain) becomes easier.

4. Long-Term Longevity Benefits:

By restoring SIRT1, PGC-1α, and FNDC5, you’re switching on the same genes activated by caloric restriction, exercise, and fasting.

Expect healthier, younger-acting muscle, and perhaps a real boost in longevity pathways.

My Dosing Protocol: How I Use SLU-PP-332

You’re probably wondering: “Okay, Hunter, how much do I take?”

Here’s exactly what I’m doing and what I recommend for most healthy, hormonally-optimized adults:

• Dose: 250–600 mcg per day (micrograms, not milligrams)

• Timing: Take in the morning (preferably fasted) to sync with your body’s natural circadian rhythm and maximize mitochondrial activation.

If you’re new to exercise mimetics, start at 250 mcg, assess your energy and recovery over a week or two, and titrate up as desired.

For the hard chargers and athletes, 500–600 mcg per day is my personal sweet spot, especially when stacking with mitochondrial support agents like BAM15 or SS-31.

Pro tip: As always, cycle your dosing. 4 weeks on, 1-2 weeks off is my general rhythm to keep receptors fresh and effects strong.

Here is the Small Molecule Cheat Sheet with some best practices.

Want to Try SLU-PP-332?

If you’re ready to test this “exercise in a bottle” effect for yourself, you can find SLU-PP-332 right now in ShredX, the all-in-one metabolic optimizer from BioLongevity Labs.

Here’s the deal:

• 20% OFF automatically until midnight PST tonight

• Stack an extra 15% OFF with code HUNTERW at checkout

Bottom Line:

If you want the mitochondrial boost, the recovery, and the muscle-optimizing edge that elite athletes fight for, SLU-PP-332 is my #1 pick for 2025.

Don’t sleep on this.

Best,

Hunter Williams