The Dark Side Of Mitochondrial Peptides

Mitochondrial health is all the rage in the biohacking world right now.

Thankfully, mitochondrial function is receiving more attention now, and people are realizing how imperative it is to overall health.

But as we descend deeper into the rabbit hole, we may eventually encounter unintended consequences that can be massively detrimental in the long run.

This was the subject of my new YouTube video today.

Increasing mitochondrial function is a worthwhile pursuit, but as with everything, the law of diminishing returns applies.

Here’s what most people DON’T realize:

• You can overstimulate your mitochondria.

• It can cause serious metabolic stress.

• And if you’re using mitochondrial stimulants, you MUST know how to manage it.

So, let’s break this down before you burn yourself out.

What Is Mitochondrial Overspin?

Your mitochondria produce energy by spinning electrons through the electron transport chain. This is how your body creates ATP (energy) and keeps you running at full speed.

But when you overstimulate your mitochondria with too many peptides, stimulants, or aggressive biohacking tactics, things can go sideways fast:

• Excess ATP production → Metabolic stress

• High reactive oxygen species (ROS) → Cellular damage

• NAD+ depletion → Chronic fatigue & burnout

• Calcium overload → Increased cell death & inflammation

In short: Too much mitochondrial activation = long-term dysfunction.

Think of it like exhaust from your car.

Having a car is great, but if too many cars are driving around, they create smog, which can choke everyone to death.

The 5 Biggest Mitochondrial Mistakes People Make

If you’re using mitochondrial stimulants like SLU-PP-332, SS-31, MOTS-C, and/or methylene blue, here are the most common mistakes you to avoid:

1. Going too hard on stimulants – If you’re stacking multiple mitochondrial activators at high doses, you’re asking for trouble in the long run.

2. Ignoring oxidative stress – High-functioning mitochondria create more ROS, which leads to inflammation, cellular damage, and premature aging.

3. Depleting NAD+ too fast – If your body burns through NAD+ faster than it can replenish, you’ll crash HARD.

4. Not cycling stimulants – Using mitochondrial activators year-round without a break wrecks your metabolic flexibility.

5. Neglecting mitochondrial uncouplers – Uncouplers act as a safety valve, preventing ATP overload and reducing ROS damage.

How to Fix Mitochondrial Overspin (Without Losing the Benefits of Mitochondrial Stimulants)

Step 1: Cycle Your Stimulants

Don’t blast SLU-PP-332, SS-31, and MOTS-C all at once and expect to thrive. A more thoughtful approach is:

• 5 days on, 2 days off

• 4-week cycles, followed by an off-phase

• Rotate different mitochondrial peptides instead of taking them all at once

Step 2: Replenish NAD+ & Antioxidants

If you’re stimulating mitochondria, you burn through NAD+ and antioxidants like crazy. You MUST replenish them.

• NAD+ injections or precursors (NMN, NR)

• Glutathione injections (100mg IM, 2-3x per week)

• Vitamin C, astaxanthin, and ubiquinol for extra protection

Step 3: Use Mitochondrial Uncouplers

Uncouplers help burn off excess energy as heat, reducing oxidative stress and preventing metabolic crashes.

In a way, they act like a filter on your exhaust pipe.

• Dihydroberberine (100-300mg per day)

• AOD 9604 (200-500mcg pre-cardio, 5x per week)

• Methylene Blue (0.5-2mg per day at LOW doses)

• Injectable L-carnitine (500-1000mg IM, pre-workout)

NOTE: these are mild uncouplers. I avoid more exotic ones, such as DNP, like the plague.

Step 4: Prioritize Recovery & Reduce Sympathetic Overload

If you’re always in fight-or-flight mode, you’re overdriving your mitochondria.

• Reduce fasting & high-intensity exercise during peak mitochondrial activation.

• Prioritize sleep & relaxation (parasympathetic activation)

• Cycle your training intensity to prevent metabolic burnout

The Year-Long Mitochondrial Plan

If you want to optimize mitochondrial function without wrecking your system, here’s a simple year-long peptide cycle:

📅 Month 1 – SLU-PP-332 (200mcg/day, 5 days on, 2 days off) + Injectable Glutathione

📅 Month 2 – MOTS-C (1mg/day, 5 days on, 2 days off) + L-carnitine (500-1000mg IM)

📅 Month 3 – SS-31 (1mg/day, 5 days on, 2 days off) + AOD 9604 (250mcg pre-cardio)

📅 Month 4 – Recovery Phase – Methylene Blue (0.5-2mg/day) + Injectable Glutathione

🔁 Repeat for 12 months.

This prevents burnout, balances ATP production and oxidative stress, enhances fat loss and metabolic flexibility, and ensures long-term cellular health.

Balance Stimulation with Protection

Mitochondrial activation is one of the most potent tools for biohacking, longevity, and peak performance… but only if it is done right.

• You STILL need to balance ATP production.

• You STILL need to prevent oxidative stress.

• You STILL need to cycle your peptides & supplements.

If you do that, you’ll get all the benefits (energy, fat loss, anti-aging) WITHOUT the crashes, fatigue, or long-term damage.

Best,

Hunter Williams

P.S. BioLongevity Labs is offering 20% off everything in the supplement category. I recommend picking up ShredX (which pairs SLU-PP-332 with AOD9604), or Shred (at 100,250, or 500mcg dosing) to optimize your mitochondrial spin!