The Easy Way To Mix A Blended Peptide

One of the most common questions I get from people new to peptides is how to reconstitute a blended peptide properly.

I’ve answered this in numerous videos and emails, but for the sake of positive reinforcement, let’s go over it again today.

Just last week, someone asked me:

This is a great question, and if you’ve ever found yourself second-guessing your math when reconstituting peptides, I’ve got you covered.

Think of It Like Lemon & Lime Water

The easiest way to understand reconstituting a blended peptide is to think about making a simple drink—let’s say lemon-lime water.

Imagine you make a big pitcher of lemon-lime water by adding five squeezed lemons and five squeezed limes into a total of two liters of water.

• The lemon and lime are two separate ingredients (just like BPC-157 and TB-500).

• When you mix them into the water, they blend evenly (just like peptides dissolve in bacteriostatic water).

• Every time you pour a small cup from the pitcher, you’re getting the same balanced mix of lemon and lime (just like every peptide dose contains both BPC-157 and TB-500 in equal amounts).

Now, let’s say you pour exactly 1/20th of the total pitcher into a small cup—that means you’re getting 1/20th of the total lemon juice and 1/20th of the total lime juice.

The same thing happens when you withdraw 10 units (0.1mL) from your reconstituted peptide vial that contains 2mL of water. Let’s keep going.

How to Reconstitute a BPC-157/TB-500 5mg/5mg Blend

When you get a BPC-157/TB-500 blend, your vial will contain 5mg of BPC-157 and 5mg of TB-500 in a dry, lyophilized powder form.

To reconstitute it, follow these steps:

Step 1: Gather What You Need

✔ 1 vial of BPC-157/TB-500 (5mg/5mg blend)

✔ Bacteriostatic Water (BAC Water)

✔ 23-gauge 3mL 1-inch needle & syringe (for withdrawing BAC water)

✔ 1mL/100-unit insulin syringe (for injecting the peptide once reconstituted)

✔ Alcohol wipes

Step 2: Add 2mL of BAC Water

For this blend, always use 2mL (200 units) of BAC water to keep dosing simple and effective.

• 2mL BAC water → 10mg total peptide (5mg BPC-157 + 5mg TB-500)

• Each 10-unit dose is 1/20th of the total vial

• That means every 10 units contains:

  • 250mcg (0.25mg) BPC-157

  • 250mcg (0.25mg) TB-500

Step 3: Reconstitute the Peptide Blend

1️⃣ Wipe the vial’s rubber stopper with an alcohol wipe.

2️⃣ Attach the 23-gauge 3mL 1-inch needle to a syringe and withdraw 2mL of BAC water.

3️⃣ Slowly inject the BAC water into the peptide vial at an angle, letting it run down the side. (Don’t squirt it directly onto the powder—it’s delicate.)

4️⃣ Let the vial sit for 5 minutes—the powder will dissolve on its own.

5️⃣ Swirl gently (DO NOT shake). Your blend is now fully reconstituted and ready to use.

6️⃣ Remove the large needle and switch to the 1mL insulin syringe for injections.

How to Dose Your Reconstituted BPC-157/TB-500 Blend

Since you now have a 2mL solution with 10mg of total peptide, every 10-unit dose is 1/20th of the entire vial.

Just like pouring 1/20th of your lemon-lime water from the pitcher, 10 units of your peptide mix gives you 1/20th of the total dose inside the vial.

Dosing Breakdown (with 2mL BAC Water Added):

✔ 10 units (0.1mL) = 250mcg BPC-157 + 250mcg TB-500

✔ 20 units (0.2mL) = 500mcg BPC-157 + 500mcg TB-500

✔ 40 units (0.4mL) = 1mg BPC-157 + 1mg TB-500

For injury healing, gut health, or general recovery, most people use:

• 10-20 units per day (250mcg-500mcg of each peptide) for maintenance and recovery

• 40 units per day (1mg of each peptide) for aggressive healing protocols

Common Reconstitution Mistakes to Avoid

🚫 Shaking the vial – This can damage the peptides. Always swirl gently.

🚫 Injecting BAC water too fast – Drip it down the vial’s side to prevent foaming.

🚫 Using too little BAC water – Higher concentrations can make dosing difficult.

🚫 Using the wrong syringe for reconstitution – A 23-gauge needle with a 3mL syringe makes withdrawing BAC water easier. Always switch to an insulin syringe for precise injections.

Final Takeaway: Reconstitution is Simple When You Understand the Basics

Just like adding lemon and lime juice into one glass of water, peptides blend together evenly in BAC water.

✔ Every dose will contain both peptides in equal amounts.

✔ Reconstitution follows the same process as a single peptide—just make sure you calculate your dose properly.

✔ Use 2mL of BAC water per vial to make dosing easy.

✔ Withdraw BAC water with a 23-gauge 3mL syringe, then use a 1mL insulin syringe for injections.

✔ Every 10-unit dose is 1/20th of the total vial, so your dose is always proportionate.

Once you understand this, you’ll never have to stress about reconstitution again.

Best,

Hunter Williams

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